He put together hundreds of Squidoo lenses for companies with major brands and promoted it as a Squidoo product, Brands in Public. In a Squidoo HQ post, Godin reported demanding $400 per month from each of the companies, even though - shades of the future - neither he nor anyone else had permission from the owners to create the pages to begin with. What did the marketing genius do to earn Young’s condemnation? “ From permission marketing fame to cyber greenmail shame, Seth Godin has finally jumped the shark,” according to internet attorney Mike Young. In a notorious initiative, Godin discarded the concept that made him famous:Īd: Baby, It’s You. Other efforts descended from obvious to ridiculous You searched for “shower curtains” and you got a Squidoo catalog of pasted in Amazon products. Seth Godin built a site where anyone with internet access could pluck products from Amazon and sell them freely, gaming the web crawlers for key word dominance. There were no restrictions on marketing excess. Seth Godin at Work Godin has emerged as a skilled writer of inspirational marketing texts, but the results haven’t matched up to the rhetoric. | Source Let’s Look at the Historyįrom inception, although Godin and his partners promoted Squidoo as a place to write about your passions, it was the go to place for marketing products on commission for affiliates, especially Amazon and eBay.Īll the self-proclaimed experts selling strategies for making money on the internet, usually with little or no effort, on autopilot, sent their customers straight to Squidoo. Wikipedia goes on to explain that fraud can be both a civil wrong and a criminal offense. “ Fraud is a deception deliberately practiced in order to secure unfair or unlawful gain.” Being impervious to one’s own inadequacies and failures, as Godin also seems to be, is perfectly legal too.īut taking control of assets belonging to others and leveraging them for your own private profit, while scheming to cut the owners out of their share, isn’t. Of course, being a klutz at marketing management, which Godin certainly is, is not a crime. The mass hates change once the story’s embedded. That Godin retains a reputation for internet marketing brilliance, even after a bumbling of Squidoo that rivals what you’d expect from a failing high school senior, shows us that the blogosphere loves hewing to the ongoing narrative as much as the mass media does. ![]() Here’s my in the middle of the wreck commentary while kicking my way through the ruins… Seth Godin Killed Squidoo: Serious Trespass of Ethics or Worse?Īlready with a history of internet irresponsibility, his actions in laying the final hammer to Squidoo may top his other strange behaviors for their cunning ability to manipulate facts for PR and trusting followers for profit. It’s grisly, but when it comes to horror stories, the death of Squidoo in 2014 was the worst. Squidoo made it personal, for better, then for worse. Good faith counts as much as contracts when you write for the web. Mark’s Square, Venice, Italy, Fine Art Photography Print/© Deborah Julian. One, Seekyt, under new ownership, continues to publish my articles, credited to a different author.īecause the world trusts WordPress, I’ve migrated all my blog content… Some vanish without warning, taking your work (and sometimes royalties) with them. If you’ve been posting as long as I have, you’ve probably seen some gross abuse by website owners. Spammers hurt Squidoo, but writers took the brunt of it… while Godin and HubPages profited.Īssorted Ideas, Large & Small Intro for Seth Godin Killed Squidoo
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The reason that coal ash ponds are dangerous is because they are packed with hazardous and carcinogenic material. That year, Indiana ranked third in the nation for total coal consumption. Most of the state’s ash ponds do not have a liner in place, meaning there is no barrier to prevent contaminants from leeching into the environment.Ĭoal provided about 58% of Indiana’s electricity net generation in 2021, according to the U.S. Then it’s transported to landfills.Īccording to PBS, Indiana has about 86 coal ash ponds and 13 landfills – more than any other state. Typically, coal ash is stored in large open ponds until it dries out. Basically, they’re a bit like a giant ashtray filled with the remains of coal that was burned by power plants to generate electricity. So, get your boots on because we’re going to get dirty.Ī coal ash pond is sometimes referred to by several names, such as an ash basin or surface impoundment. We’re going to take a dive into some of the nastiest details that we can dig up to keep our readers informed about what coal ash ponds are, why they’re so toxic, and what kinds of things are being done to rehabilitate these locations. doi:10.Coal ash pond cleanups have become a major environmental topic throughout the country lately, and lots of hazardous work on these sites has been taking place right here in Indiana. ![]() Investigation of the anti-inflammatory potential of Aloe vera gel (97.5%) in the ultraviolet erythema test. ![]() Reuter J, Jocher A, Stump J, Grossjohann B, Franke G, Schempp CM. Moisturizing effect of cosmetic formulations containing Aloe vera extract in different concentrations assessed by skin bioengineering techniques. Penn Medicine.ĭal'Belo SE, Gaspar LR, Maia Campos PM. Do Face Masks Really Work? The "Magic" Behind Face Masks - and How to Get the Most Out of Them. The Trustees of the University of Pennsylvania. A systematic review study of therapeutic effects of Matricaria recuitta chamomile (chamomile). Biological and pharmacological activities of squalene and related compounds: potential uses in cosmetic dermatology. A study to assess the effect of a cream containing farnesol and xylitol on atopic dry skin. A novel method to control the balance of skin microflora Part 2. Effects of isoflavones from red clover (Trifolium pratense) on skin changes induced by ovariectomy in rats. ![]() An Evaluation of Efficacy and Tolerability of Novel Enzyme Exfoliation Versus Glycolic Acid in Photodamage Treatment. Use of silicon for skin and hair care: An approach of chemical forms available and efficacy. Cosmeceuticals for Hyperpigmentation: What is Available? J Cutan Aesthet Surg. Antioxidant and potential anti-inflammatory activity of extracts and formulations of white tea, rose, and witch hazel on primary human dermal fibroblast cells. International Journal of Cosmetic Science. The effect of physically applied alpha hydroxyl acids on the skin pore and comedone. Comparative Study of the Biological Activity of Allantoin and Aqueous Extract of the Comfrey Root. Stabilization of the triple helix in collagen mimicking peptides. ![]() Salicylic acid as a peeling agent: a comprehensive review. Our testers used these products for weeks at a time, taking note of how well each clarified their skin and improved overall pore appearance. We considered dozens of products and board-certified dermatologists helped narrow down our selection.Īhead, find the best pore minimizers out there today.įlament F, Francois G, Qiu H, et al. To find the very best in pore minimizing, we conducted hours of research on the best pore minimizers and tried out some of the top-rated products on the market, from toners to exfoliating treatments. "It also reduces excessive oil production, preventing clogged pores in the first place." "Salicylic acid exfoliates the skin and also penetrates the pores to dissolve the dead skin cells and debris that may be clogging them," Dr. Whether it's a toner, serum, moisturizer, or mask, she recommends products containing retinoids, niacinamide, AHAs (alpha-hydroxy acids), and salicylic acid (beta-hydroxy acid or BHA). "Ingredients that are ' pore minimizers' simply help pores appear smaller by exfoliating your skin, unclogging pores, and preventing sun damage," says board-certified dermatologist Dr. That said, we know of some standout skincare formulations that can make them a bit less glaring. Pores are just a fact of life, and no product or procedure will effectively get rid of their appearance. ![]() The weighted feel is meant to help soothe your child even if you aren’t right next to the crib or bassinet to do it with your touch.The 100% cotton is washer and dryer safe, so you won’t have to worry about shrinkage or dealing with a misshapen swaddle blanket after washing it.There is an inner pocket for baby legs to slide into for the first few weeks and then a larger inner pocket to accommodate your baby’s legs for several months as he or she grows.You can buy it on the manufacturer’s website and Amazon or in-store at Buy Buy Baby. That’s not to say that it’s the only swaddle blanket designed in such a way, but it does add to its ease of use. Swaddle blankets, in general, are easy enough to get the hang of, but with the Nested Bean Zen Swaddle, you also get one that’s already shaped to show you the best way to fold the different flaps, as opposed to one large square of fabric, which can sometimes be confusing. And, thanks to the 100% lightweight cotton, the fabric is breathable enough for the warmer months of the year, yet still warm enough to keep your baby nice and toasty during the colder months. The weights themselves are non-toxic poly bends that are also used at some children’s toys, so there’s no real danger there. The chest and sides of the blanket are gently weighted to give your baby the feeling of being held, even after you’ve put him or her back in the crib or bassinet. What does set it apart from some other similar swaddle blankets, though, is the fact that it’s actually lightly weighted. The Nested Bean Zen Swaddle is one that is designed for babies up to six months old, so you can be sure that you’ll get plenty of use out of it while figuring out exactly how your particular baby needs to be swaddled each time. And depending on your baby, swaddle blankets could be the key to getting him or her to sleep through the night more easily while keeping them comfortable. They’re typically inexpensive and, for the most part, easy to use. Tommee Tippee vs Avent: Which is Better? - January 5, 2023Ī swaddle blanket is one of those things that your baby will invariably need right from the start.Baby Formula Recalls : Latest Baby Formula Recall - January 16, 2023.Brown vs Avent Bottles: Which is Better? - January 23, 2023 Tests have been performed in leading safety laboratories and inspected by pediatric pulmonologists for breathing safety. The gently weighted Zen Swaddle exceeds rigorous safety standards, both mandatory and voluntary, by over 8 times. Please view the size chart to make sure your baby is the correct size for the gently weighted Zen swaddle. Designed to help them sleep through the Moro reflex stage. The gently weighted Zen Swaddle™ echoes your reassuring touch on a baby’s chest and sides, so your little one always feels comforted and secure. ![]() And just like that Sleep got sweeter and everyone slept happily ever after. ![]() From there Nested Bean has continued to grow with your baby. Then getting the thumbs up from the labs and parent tester which then led to the first ever gently weighted swaddle and Nested Bean was born. This got her thinking and led to tons of research and consulting with doctors and pediatric pulmonologists. Nested Bean was founded when a sleep deprived mom replaced her hand on her son's chest with a little stuffed toy and he stayed sleeping under its gentle pressure. You may lose slightly more ground this way but the huge gains you make when you guess correctly will more than make up for this. If you guessed wrong, simply move to the other side immediately, like you would anyway. You will naturally sometimes guess wrong as the fish can change direction quickly, but when this happens Kisara can change directions very swiftly herself, so quickly press the opposite button to recover, before guessing again for next time.įor instance, lean to the right and if the directional prompt also goes right you will gain a large bonus that will massively push you towards the green. ![]() There is no penalty for doing this unless you linger on the wrong direction after a prompt has appeared. The key here is to always have Kisara pulling one side or the other, and never leave her in a neutral position. Once I cottoned onto this, I can honestly say I never ever missed a fish. With the basics covered, here’s how to actually succeed in catching a fish every time. Tales of Arise fishing tips to catch fish every time The only thing you lose is your opportunity. Note that there is no punishment for failing – if a fish gets away at any point, you don’t expend any items. If your own health bar runs out first, you’ll lose the fish. If the health bar of the fish is depleted entirely before your line’s health bar, you’ll successfully catch it. Get it wrong, or fail to press in time and your health bar will take a hit. When directions appear, holding down the signified direction will reduce the fish’s health bar and boost your line’s health in the progress.Įvery now and then, the camera will slightly zoom in towards the fish which is where the shoulder button prompts appear, considerably depleting the fish’s health if you hit it in time. The prompts will demand you either hold a directional button, which are always left or right, or perform one-off shoulder-button presses. Your line’s health bar will deplete gradually over time but can be restored when you correctly press certain buttons when prompted. Your line’s health bar will appear near Kisara, along with the fish’s own health bar within the water. When the dramatic music kicks in, things start to get serious. ![]() You’ll often still somewhat attract a fish. It also doesn’t necessarily matter if you keep mistiming and pressing outside of the circular target. Try a few combinations and see what draws in the biggest crowd. Sometimes you’ll need a soft shake followed by a hard shake. Now here’s the thing that isn’t really explained that well – there are specific combinations that lure certain fish. Once a fish reaches the line on the left hand side, you’ve snagged them onto your bait and move onto the next phase. ![]() If you press the button when the ring is within the target, it will cause a bigger reaction and potentially attract the fish closer. Pressing again will cause the fish to react, potentially drawing them closer to the bait. Pressing one of these buttons once will cause a ring to appear and move towards a circular target. You’ll have to press buttons to perform a soft shake, a hard shake or reel in your line.Įvery fish has specific buttons and baits to lure them, so you will have to decide whether to start with a soft shake, hard shake or just reel in. Once your line is cast, a new screen will show a series of fish that can be lured to your bait along a line where the fish start on the right hand side. The ones you’ve caught previously will be highlighted in green. Cast with R2 in these spots to attract the most fish, though it’s worth noting that you can actually catch fish elsewhere and sometimes this may even be preferable if you are after a specific fish and don’t want others getting in your way.īefore casting, you can change your rod and lure with triangle – doing this will also bring up the list of fish available in the fishing spot. ![]() Move the cursor to one of these spots and it will change from blue to a yellow/golden colour. When Kisara looks out into the water, you will see bubbly areas along with occasional fish jumping out of the water. There are three main phases to fishing in Tales of Arise, which are fully explained in turn. This is unlocked automatically so don’t worry if you have spotted blatant fishing locations throughout your travels but been unable to interact with them. What are the rewards for fishing in Tales of Arise? How to unlock fishing in Tales of Ariseįishing is first introduced when the party encounters the Fishing Expert on Talka Pond Road, just after passing Viscint and acquiring all six playable party members. ![]() We are a proud member of the American Boer Goat Association and we strive to raise only healthy and high quality Boer Goats by obtaining and selecting only the best genetics in the. StarShine Meadows is a family operated farm located in Kearney Missouri just a few miles North of Kansas City. kubectl docker image We have Boer goats for sale, breeding and show stock. All animals must be picked up within that week unless other arrangements are made. A larger amount will be required to hold more than one animal. We currently do no have any available for sale. Garden & House Joplin 30 $We can offer a discount for multiple animals. Also have 2 dairy does for sale one is a year old the. For any other inquiries on how to place your order, price per livestock or shipment cost, do not hesitate to contact us.1 dairy goat buck for sale 2 months old bottle baby.$30.00. We have a lot of goats so tell us what you are looking for and maybe we can put a …White saanen goats for sale Sale! White saanen goats for sale $ 120.00 – $ 320.00 Once you submit your order, full details for payment will be sent to you by email or sent via text using the phone number you are to provide. detroit dd13 delta p sensor We normally have some Kiko goats for sale including Does, Doelings, Bucks, Bucklings and kids. The snake may be greenish, black, olive or dark brown. Goat chick's and pig (Jane …Nigerian Dwarf Goat for Sale in Missouri - Current List of Missouri Nigerian Dwarf Goat Breeders RJK Farm Ridgley's Dairy Goats Bonita Paradise Hisaw Hills Coat Of Arms Busy Browsers Red Horse Valley, LLC Winters, Katherine Rolla, MO (573) 364-4207 Nigerian Dwarf Goat Resources in Missouri AssociationsGarter snakes in Missouri are typically dark colored and have orange, yellow or white stripes that run lengthwise down the body of the snake. fayetteville, AR > for sale > farm & garden - by owner. No emails Call or text if interested NO CODES we or located in Jane Missouri we can meet ADGA Registered Nigerian Dwarf buck $250 Potbelly sow she is bred to a Registered kunekune $150 Black Cooper. Just e-mail us call us or use the contact page to schedule a farm visit to come and check them out. If you can use a young buck in your breeding program, please check with us, we will be taking more pictures soon. back and better amira wattpadPygmy Goat Babies ~ Registered Nigerian Dwarf Goat/Mini donkey. Selling high quality spotted and colored Boer Goats. Essary Boer Goats, Rogersville, Missouri. I feed alfalfa to the girls and brome or orchard for the boys. They need good hay, especially in the winter. They CAN NOT live on just grass, weeds or brush. If you don’t have a barn or shed a calf hut with a half opening with the opening turned away from wind will suffice for dwarf goats. Layman, a cattle dairyman, retired from dairying and turned to raising Boer goats nearly two decades ago.Goats NEED a shelter to get out of rain, wind, and snow. tamilblasters alternative All About Essary’s Green Acres Layman and Judy raise high quality fullblood Boer goats on their farm in the Missouri Ozarks. They grow rapidly, are somewhat angular in appearance, prolific and tolerant of rustic conditions. They have been in the United States only since about 1995. The Kiko was developed in New Zealand to create a more muscular and productive indigenous goat. The Kiko is an aggressive forager, capable of thriving under conditions of feed deprivation. ![]() Pick up only, Patterson MO do NOT contact me with unsolicited services or offers post id: 7581408945Goats NEED a shelter to get out of rain, wind, and snow. One blue eyed buck, all other adult does are does, 2 doelings, 3 bucklings. affordable modular sofa reddit Goat herd - $2,200 © craigslist - Map data © OpenStreetMap Goat herd Nigerian Dwarf/ Pygmy mix. Columbia, MO for sale "Goats" - craigslist. ![]() Delete duplicates in just a few clicks.Navigate through your entire photo collection.Choosing all the right resolution, sharpening and compression settings.Adjust white balance, Frame, rotate, flip and frame pictures.Automatically fix small imperfections and correct the red-eye effect, Video editor Business, coming from the developer Movavi, is running on Android systerm.A fully featured toolbox of photo editing tools.Enhance photos automatically or under your supervision,.Remove objects such as distracting shadows, power poles or simply random passers-by.Smart Select, Smart Mask, and Smart Background.Aplikasi ini dikembangkan oleh Movavi yang terintegrasi update sampai pada Agustus tahun 2022. Clean up distractions in your photos with a click of a button! 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With these easy tools, you can automatically fix small imperfections and correct the red-eye effect, control brightness and contrast, adjust white balance, as well as the frame, rotate, flip and frame pictures to achieve the best composition. This tool has been equipped with a fully featured toolbox of photo editing tools. SoftOrbits Photo Editor Pro will help you select the whole object and re-create what’s beneath it, producing a clean, perfect postcard free of blemishes and distractions. SoftOrbits Photo Editor Pro Review at a Glance SoftOrbits Photo Editor Pro Review at a Glance.Movavi Picverse 1.6 ( 圆4) Multilingual.Movavi Picverse 1.7 ( 圆4) Multilingual.Movavi Picverse v1.7 ( 圆4) Multilingual.Movavi Picverse 1.9.0 Multilingual Portable.Movavi Picverse 1.9.1 Multilingual Portable details 'MovaviPicverse1.1.0.exe' wrote 4 bytes to a remote process 'TEMPis-TIBT7.tmpMovaviPicverse1.1.0.tmp' (Handle: 168) 'MovaviPicverse1.1.0.exe' wrote 32.Movavi Picverse 1.10 ( 圆4) Multilingual. ![]() Movavi Picverse 1.11 ( 圆4) Multilingual.Movavi Picverse v1.11 ( 圆4) Multilingual.Movavi Picverse 1.1 ( 圆4) Multilingual Portable.Movavi Picverse 1.6 ( 圆4) Multilingual Portable.Movavi Picverse 1.11 ( 圆4) Multilingual Portable. ![]()
![]() I was working all the time but I hadn’t done shows or real editorial work. I was working with a well-known hairdresser, Yannick d’Is, on a French beauty campaign and had a bob down to my chin. VALLETTA: Nobody had short hair at the time. INTERVIEW: What prompted the new haircut? I was about 18 when I cut my hair off-the little pixie haircut. Then I cut my hair short and it basically changed everything overnight. The other big break would have been a cover of French Elle with Hans Feurer. I think I had one or two test shoots there. That was two weeks after being in Milan for the very first time. I cried on set because I didn’t know what to do. VALLETTA: It was an advertorial for Italian Vogue. INTERVIEW: What was your first really big job? It was like the call of the wild-I just couldn’t not go anymore. But my career was starting and people were calling and I kept getting pulled out of school to work anyway. In retrospect, I wish I had spent one more year at home. Luckily, I had already traveled to Europe twice before I finally moved out at 17. I didn’t even really know what that meant. They asked if I wanted to come to Europe for the summer to model. ![]() My mom had paid for modeling classes, and some scout came through and discovered me. VALLETTA: I got discovered in Tulsa at a local agency. INTERVIEW: How did you get from Tulsa to Europe? INTERVIEW: You grew up in Oklahoma, right?ĪMBER VALLETTA: I grew up in Tulsa, then moved to Europe to model when I was 17. Amber VallettaĪBOVE: AMBER VALLETTA IN NEW YORK, JULY 2013. ![]() Two guys cutting my hair at the same time. They looked at me and said, ‘Let’s cut your hair off.’ We went back to my apartment in Paris and just cut it all off. #fuses NODEBUG, USBDIV, PLL5, CPUDIV1, VREGEN, CCP2B3 #fuses HSPLL, NOMCLR, PUT, BROWNOUT, BORV43, NOWDT, NOPROTECT, NOLVP Pin C6 (25) is directly assigned to pin TTL-Rx in Maestro.Ĭrystal oscillator: 10MHz with 22pF capacitors. I took them making 2 jumpers (orange wires) on servo bus. The same +5V applied to PIC18F4550 is applied to Maestro. Putc(0x70) // Target position = 1500 us (typical neutral for servos)Īs you can see the attached images, I’m using Micro Maestro. Send another Set Target command using the Pololu protocol. Putc(0x20) // Target position = 1000 us (typical minimum for servos) Send a Set Target command using the Pololu protocol. When a power the circuit, microMaestro takes 2 seconds to blink continuously red LED, so I guess something is wrong. I made only this modification on C code: #use delay(clock=10000000) I connected a 10MHz oscillator crystal with two 22pF capacitors. Vin is powered in +5V (the same power source of PIC18F) and both PIC18F and microMaestro are grounded properly. I really need some kind of intensive help, for this.Īs mentioned in manual, I connected pin 25/Tx/RC6 of PIC18F4550 into Rx MicroMaestro. ![]() I checked the communication and all connections, but I can’t find what is going wrong. I’ve tried to compile the PIC C code example in manual, but simply nothing happens. I think the schematics seems like as mentioned in manual. The servo 1 moves again, for column two, and then servo 2 moves 25 times to fill the second row… And so on.Ĭould you help me to write a C code for PIC18F4550, for this application? ![]() ![]() So, while the first one move one step, 4 degrees (25 movements x 4 degrees = 100 degrees of range), which represents the first column, the second servo moves 25 times to fill the first row (4 degrees too). The servos are combined (arranged) to move in rows and columns. I need to control two servos, to make a 25x25 matrix movement. Could you please explain me what the C code for PIC18F4550 in manual really do for one servo? What I am a little confused is about the Hex codification sent through putc command. ![]() Despite these advances, most of the techniques still require either substantial programming skills or significant manual correction of errors, and we are unaware of any method capable of computing all parameters of interest in one single analysis with little manual effort. The available methods to date can compute fiber, capillary, myonuclei and satellite cell counts, fiber type, and centrally located myonuclei, as well as fiber morphometric parameters such as cross-sectional area (CSA) and Feret diameter or perimeter ( 2, 6, 15, 16, 18, 23, 24, 27, 30, 35, 41). Once the settings are customized for the specific samples of interest and high-quality outputs are confirmed, automated methods can perform the analyses in an unbiased manner, which arguably represent an improvement over the manual method. To overcome these issues, a variety of semiautomatic and fully automatic approaches to analyze muscle immunohistochemical images have been developed. This manual work may suffer from low reproducibility and risk of bias due to the subjective nature of the analysis procedure ( 3, 5). Unfortunately, immunohistochemical image analysis is a time-consuming process that requires extensive manual effort. Immunohistochemistry applies antibodies and immunofluorescent labeling on muscle cross-section, which, with the use of immunofluorescence microscopy, allows for visualization and subsequent quantification of the targeted morphological parameters. Although several methodological approaches have been used to study these adaptive responses at the muscle fiber level, one of the most commonly used techniques is immunohistochemistry. Skeletal muscle is a plastic tissue that rapidly adjusts to external demands and environmental stimuli such as exercise, inactivity, and disease ( 9, 13). Thus, the Muscle2View is a viable tool for researchers aiming to quantify immunohistochemical variables from skeletal muscle biopsies. In addition to fiber typing, myonuclei counting, and the quantification of fiber type-specific morphological measurements, the Muscle2View pipeline can identify the complex capillary-to-fiber network from a batch of images within minutes. NEW & NOTEWORTHY Here, we developed a freely available CellProfiler-based pipeline termed Muscle2View, which provides unbiased, high-content analysis of muscle cross-sectional immunohistochemistry images. This robust analysis is done in one single run within a user-friendly and flexible environment based on the free and widely used image software CellProfiler. In addition to the classical morphological measurements, the Muscle2View can identify the complex capillary-to-fiber network and myonuclear density in a fiber type-specific manner. Collectively, we demonstrate that the Muscle2View pipeline can provide unbiased and high-content analysis of muscle cross-sectional immunohistochemistry images. For several variables, however, there were differences (5–15%) between values computed by manual counting and Muscle2View, suggesting that the methods should not necessarily be used interchangeably. When comparing the Muscle2View pipeline to manual or semiautomatic analysis, overall the results revealed strong correlations. The novel identification of the capillary-to-fiber interface allowed for the calculation of microvascular factors such as capillary contacts (CC), individual capillary-to-fiber ratio (C/Fi), and capillary-to-fiber perimeter exchange (CFPE) index. ![]() Provided that the images are of sufficient quality and the settings are configured for the specific study, the pipeline allows for automatic and unsupervised analysis of fiber borders, myonuclei, capillaries, and morphometric parameters in a fiber type-specific manner from large batches of images in <10 min/tissue sample. Here, we developed Muscle2View, a free CellProfiler-based pipeline that integrates all key fiber-morphological variables, including the novel quantification of the capillary-to-fiber interface, in one single tool. Because manual immunohistochemical analysis of features such as skeletal muscle fiber typing, capillaries, myonuclei, and fiber size-related parameters is time consuming and prone to user subjectivity, automatic computational methods could allow for faster and more objective evaluation. |